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KMID : 0900920010250010001
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Korean journal of Animal Reproduction
2001 Volume.25 No. 1 p.1 ~ p.7
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Establishment of Bovine Ovum Bank : I. Full Term Development of Vitrified In Vitro Matured Hanwoo (Korean Cattle) Oocytes by Minimum Volume Cooling (UC) Method
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Kim E.-Y.
Kim D.-I.
Rhee M.-G.
Weon Y.-S.
Nam H.-K.
Lee K.-S.
Park S.-Y.
Park E.-M.
Yoon J.-Y.
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Abstract
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This study was to test whether in vitro matured Hanwoo oocytes can be successfully cryopreserved by a new vitrification procedure using MVC method. For the vitrification, oocytes were pretreated in 10% ethylene glycol (EG10) for 5~10 min, exposed in EG30 for 30 sec, each oocyte was individually put on the inner wall of 0.25 mell straw, and then straws were directly plunged into LN_2. Thawing was taken by 4-step procedures 〔1.0 M sucrose (MS), 0.5 MS, 0.25 MS, and 0.125 MS〕 at 37^{circ}C. In vitro developmental capacity (survival, cleavage (geq2-cell) and blastocyst rates) in vitrified group was no significant difference compared to that in other treatment groups (exposed; 100.0, 74.4, 32.3% and control; 100.0, 78.3, 36.3%): high mean percentage of oocytes (91.2%) was survived, 69.4% of them were cleaved and 27.9% of cleaved embryos were developed to blastocyst. Especially, after transfer of in vitro developed embryos in vitrified group, four of six recipient animals were pregnant and three of them were ongoing-pregnant by manual palpation at 250 days after transfer. This result demonstrates that MVC method is very appropriate freezing method for the Hanwoo in vitro matured oocytes and that ovum bank can be maintained efficiently by MVC cryopreservation method.
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KEYWORD
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Bovine in vitro matured oocyte, Vitrification, Thawing, in vitro survival, Full term development
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